Cartridges and plate for Glycan Purification: LudgerClean EC50 range
Ludger’s glycan purification cartridges (catalogue no. LC-EC50-24) and plate (catalogue no. LC-EC50-96) have been designed to purify glycans from non-carbohydrate material including salts, proteins and detergents by electronic interaction of the glycans with the surface of the cartridge
Clean-up of glycans can be performed using EC-50 cartridges/plate:
- after enzymatic or chemical release of glycans from glycoproteins
- after exoglycosidases (enzymatic) digestion of glycans to release individual monosaccharides to confirm glycan identity and structure
- before and after glycan labelling using fluorescent tags such as 2-aminobenzamide acid (2-AB) or 2-aminobenzoic acid (2-AA)
Workflow:
LC-EB10 and LC-EC50 Comparison Table:
A wide range of glycans including N-linked and O-linked type oligosaccharides, tri-saccharides and larger structures can be purified using LC-EC50-24 cartridges or LC-EC50-96 plate as depicted in the comparison table and can be further analysed using orthogonal techniques.
To view a table outlining our full range of LudgerClean products available, click here
Product/Ordering Information:
Release monosaccharides and label with 2AA:
Companion Products:
- LP-COLLPLATE-LID-96
- Collection Plate Lid, 5 per pack (can be used with LC-EC50-96)
Release Kits (Enzymatic and Chemical):
- LZ-rPNGaseF-kit
- LudgerZyme recombinant PNGase F kit
- E-PNG01
- PNGase F (Peptide N Glycosidase F)
- LL-HYDRAZ-A2
- Ludger Liberate Hydrazinolysis N- and O-glycan release kit
Labelling Kits:
- LT-KAB-A2
- LudgerTag 2-AB glycan labelling kit, sodium cyanoborohydride reductant
- LT-KAB-VP24
- LudgerTag 2-AB glycan labelling kit, 2PB reductant
- LT-KAB-VP96
- LudgerTag 2-AB glycan high throughput labelling kit, 2PB reductant
- LT-KAA-A2
- LudgerTag 2-AA glycan labelling kit, sodium cyanoborohydride reductant
- LT-KAA-VP24
- LudgerTag 2-AA glycan labelling kit, 2PB reductant
- LT-KPROC-VP24
- LudgerTag Procainamide glycan labelling kit, 2PB reductant
- LT-KPROC-24
- LudgerTag Procainamide glycan labelling kit, sodium cyanoborohydride reductant
- LT-KPROC-96
- LudgerTag Procainamide glycan labelling kit, sodium cyanoborohydride reductant
Please contact us with any questions or for a quote: [email protected]
Contacts
Technical Advice
Dr. Conception Badia Tortosa
Senior Scientist
[email protected]
Quotations and Orders
Sales Team
Quotations: [email protected]
Orders: [email protected]